Research Projects on Tools for Detection

RESEARCH PROGRAMS

Tools for Detection 2007 projects (pdf file containing summaries) Comparison of Dry Sheet Media and Conventional Agar Media Methods for Enumerating Yeasts and Molds in Food (L. R. Beuchat, D.A. Mann, and J.B. Gurtler) *Surface-sterilization Methods for Escherichia coli* O157:H7 on Lettuce (Lactuca sativa L.) (G. Zhang, L. Ma, L.R. Beuchat, M.C. Erickson, V.H. Phelan, and M.P. Doyle) Isolation of Yersinia pestis from Food Using Immunomagnetic Beads Coated with Monoclonal Antibody 6B5 (T. Zhao, P. Zhao, and M.P. Doyle) Silver Nanorod Array as a SERS substrate for Foodborne Pathogenic Bacteria Detection (H.-Y. Chu , Y.W. Huang, and Y.-P. Zhao) 2006 projects (pdf file containing summaries) Evaluation of Direct Plating Methods to Enumerate Alicyclobacillus in Beverages (M. B. Murray, J. B. Gurtler, J.-H. Ryu, M. A. Harrison, and L. R. Beuchat) Cryotolerance, Attachment, and Recoverability of Escherichia coli O157:H7 and Selected Surrogates from Romaine Lettuce Leaf Surfaces (M.A. Harrison) Silver Nanorod Array as a SERS Substrate for Escherichia coli O157:H7 Detection** (H.Y. Chu, Y. Liu, Y. Huang, and Y. Zhao) A Monoclonal Antibody that Binds Surface Non-Fraction 1 Antigens of Yersinia pestis (T. Zhao, P. Zhao, M.P. Doyle, D. Gottfried, and J. Xu) 2005 projects (pdf file containing summaries) *Performance of Media for Recovering Stressed Cells of Enterobacter sakazakii* as Determined Using Spiral Plating and Ecometric Techniques** (J. B. Gurtler and L. R. Beuchat) Production and Characteristics of Monoclonal Antibodies Against Surface Non-fraction 1 Antigens on Yersinia pestis (T. Zhao, P. Zhao, J. Xu, D. Gottfried, and M. P. Doyle) *Green Fluorescent Protein Labeling of Listeria* and Salmonella for Food Safety-Related Studies (G. Zhang, L. Ma, and M. P. Doyle) Molecular Detection and Serotyping of Infectious Bronchitis Virus from FTA^Ò Filter Paper (H. Moscoso, E. O. Raybon, S. G. Thayer, and C. L. Hofacre) Relationship of Messenger RNA Reverse Transcriptase-Polymerase Chain Reaction Signal to Campylobacter spp. Viability (K. D. Sung, N. J. Stern, and K. L. Hiett) DNA Isolation from Noncultivable Campylobacter jejuni Isolates (K. A. Callicott, N. J. Stern, and K. L. Hiett) Development of Rapid Detection Technique for Pathogens using Nanorod-based Sensor (Y. W. Huang, Y. Zhao, and W. Kisaalita) 2004 projects (pdf file containing summaries) An inter-laboratory evaluation of selective media for the detection and enumeration of yeasts in blue-veined cheese (B. C. Viljoen, A. Knox, L. R. Beuchat, T. Deak, M. Malfeito-Ferreira, T. K. Hansen, A. Hugo, M. Jakobsen, V. Loureiro, A. Lourens-Hattingh, and R. Vasdinnyei) 2003 projects (pdf file containing summaries) Development of a DNA Microarray Chip for Identification of Listeria species, Partial Serotyping and Assessment of the Virulence Potential of Listeria monocytogenes Isolates** (L. Ma, G. Zhang, and M. P. Doyle) A Solid Agar Overlay Method for Recovery of Heat-Injured Listeria monocytogenes (Z. Yan, J. Gurtler, and J. L. Kornacki) *Comparison of USDA Culture Procedure and Polymerase Chain Reaction (PCR) Detection of Listeria monocytogenes* on Deli Meats (C.-M. Lin, L. Zhang, and M. P. Doyle) Direct Microscopic Observation and Viability Determination of Campylobacter jejuni on Chicken Skin (W. Chantarapanont, M. Berrang, and J. F. Frank) Application of Nested PCR to Detection of Salmonella in Poultry Environment (J. J. Maurer) Detection of Salmonella and Campylobacter in Poultry by PCR-ELISA (J. J. Maurer) A Restriction Fragment Length Polymorphism Based Polymerase Chain Reaction as an Alternative to Serotyping for Identifying Salmonella Serotypes (J. J. Maurer) Rapid Detection of Poultry Salmonella enterica Serotypes Enteritidis, Hadar, Heidelberg , and Typhimurium by Multiplex PCR to Common O and H Antigen Genes and Alleles (J. J. Maurer) Inoculation Strategies and Parasite Recoveries from Experimentally Spiked Produce (C. Tatum and Y. R. Ortega) Development of a Method to Quantify Extracellular Carbohydrate Complexes Produced by Escherichia coli O157:H7** (J.-H. Ryu and L. R. Beuchat) Survival and Recovery of Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes on Lettuce and Parsley as Affected by Method of Inoculation, Time Between Inoculation and Analysis, and Treatment with Chlorinated Water (M. M. Lang, L. J. Harris, and L. R. Beuchat) *Influence of Variations in Methodology on Populations of Listeria monocytogenes* Recovered from Lettuce Treated with Sanitizers** (A. B. Burnett, M. H. Iturriaga, E. F. Escartin, C. A. Pettigrew, and L. R. Beuchat) Evaluation of Inoculation Method and Inoculum Drying Time for Their Effects on Survival and Efficiency of Recovery of Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes Inoculated on the Surface of Tomatoes (M. M. Lang, L. J. Harris, and L. R. Beuchat) *Effect of Inoculum Size, Relative Humidity, Storage Temperature, and Ripening Stage on the Attachment of Salmonella* Montevideo to Tomatoes and Tomatillos (M. H. Iturriaga, E. F. Escartin, L. R. Beuchat, and R. Martinez-Peniche) Factors Affecting Survival, Growth, and Retrieval of Salmonella Poona on Intact and Wounded Cantaloupe Rind and in Stem Scar Tissue** (L. R. Beuchat and A. J. Scouten)