1109 Experiment St. - Melton Building - Griffin, GA 30223 USA - PH (770) 228-7284 - FAX (770) 229-3216
Dr. Michael Doyle
Regents Professor of Food Microbiology
Director, Center for Food Safety

Center for Food Safety
Dept. Food Science & Technology
University of Georgia
Griffin, GA 30223-1797
Office Phone: (770) 228-7284
E-Mail: mdoyle@uga.edu

Overview of Research Program
Dr. Doyle's research is in the area of food microbiology and focuses on bacterial foodborne pathogens.
Pathogens under study include Escherichia coli O157:H7 and other serotypes of enterohemorrhagic E. coli, Salmonella spp., Campylobacter jejuni, Listeria monocytogenes, Staphylococcus aureus, and Clostridium botulinum.
Development of probiotic/competitive exclusion bacteria to reduce/eliminate carriage of E. coli O157:H7 by cattle and Campylobacter jejuni by poultry.
Development of treatments using approved chemicals to kill foodborne pathogens on produce, meat and poultry.
Development of methods to isolate Helicobacter pylori from foods.
Studies on the infectious dose of Listeria monocytogenes
Studies on the ecology of Salmonella typhimurium DT104
 
Patents

Doyle, M. P., T. Zhao, B. G. Harmon, and C. A. Brown. November 5, 2003. Control of enterohemorrhagic E. coli O157:H7 in cattle by probiotic bacteria. China Patent No. ZL 98808111.3.

Doyle, M.P. and T. Zhao. March 17, 2009. Composition and method for inhibition of microorganisms. European Patent Office # P713476EPPCT/DE.

Doyle, M.P. and T. Zhao. August 18, 2009. Composition and method for inhibition of microorganisms. U.S.A. Patent # 7,575,744.

Doyle, M. P., T. Zhao, B. G. Harmon, and C. A. Brown. January 10, 2002. Control of enterohemorrhagic E. coli O157:H7 in cattle by probiotic bacteria. New Zealand Patent No. 502420.

Doyle, M. P., T. Zhao, B. G. Harmon, and C. A. Brown. October 12, 1999. Control of enterohemorrhagic E. coli O157:H7 in cattle by probiotic bacteria. U.S.A. Patent #5,965,128.

Publications (2008 - present)
 

Doyle, M.P., M.C. Erickson, W. Alali, J. Cannon, X. Deng, Y. Ortega, M.A. Smith, and T. Zhao. 2015. Food industry's current and future role in preventing microbial foodborne illness within the United States. Clin. Infect. Dis. doi:10.1093/cid/civ253. In Press.

Erickson, M.C., L.M. Ma, and M.P. Doyle. 2015. Clostridium botulinum toxin production in relation to spoilage of Atlantic salmon (Salmo salar) packaged in films of varying oxygen permeabilities and with different atmospheres. J. Food Prot. In Press.

Erickson, M.C., C. Smith, X. Jiang, I.D. Flitcroft, and M.P. Doyle. 2015. Manure source and age affect survival of zoonotic pathogens during aerobic composting at sublethal temperatures.  J. Food Prot. 78:302-310

Erickson, M.C., C. Smith, X. Jiang, I.D. Flitcroft, and M.P. Doyle. 2015.  Survival of Salmonella or Escherichia coli O157:H7 during holding of manure-based compost mixtures at sublethal temperatures as influenced by the carbon amendment.  J. Food Prot. 78:248-255.

Chen, D., T. Zhao, and M.P. Doyle. 2014. Transfer of foodborne pathogens during mechanical slicing and their inactivation by levulinic acid-based sanitizer on slicers.  Food Microbiol. 38:263-269.

Donado-Godoy, P., V. Clavijo, M. Leon, A. Arevalo, R. Castellanos, J. Bernal, M. Tafur, M.V. Ovalle, W.Q. Alali, M. Hume, J.J. Romero-Zuniga, I. Walls, and M.P. Doyle. 2014. Counts, serotypes, and antimicrobial resistance of Salmonella isolates on retail raw poultry in the People's Republic of China. J. Food Prot. 77:894-902.

Erickson, M.C., M.Y. Habteselassie, J. Liao, C.C. Webb, V. Mantripragada, L.E. Davey, and M.P. Doyle. 2014. Examination of factors for use as potential predictors of human enteric pathogen survival in soil. J. Appl. Microbiol. 116:335-349.

Erickson, M.C., J. Liao, X. Jiang, and M.P. Doyle. 2014. Contribution of chemical and physical factors to zoonotic pathogen inactivation during chicken manure composting. Agriculture, Food and Analytical Bacteriology 4:96-108.

Erickson, M.C., J. Liao, L. Ma, X. Jiang, and M.P. Doyle. 2014. Thermal and non-thermal factors affecting survival of Salmonella and Listeria monocytogenes in animal manure-based compost mixtures. J. Food Prot. 77:1512-1518.

Erickson, M.C., C. Smith, X. Jiang, I.D. Flitcroft, and M.P. Doyle. 2014. Survival of Salmonella enterica and Listeria monocytogenes in manure-based compost mixtures at sublethal temperatures. Agricult. Food Anal. Bacteriol.  4:224-238.

Erickson, M.C., J. Liao, X.P. Jiang, and M.P. Doyle. 2014. Inactivation of pathogens during aerobic composting of fresh and aged dairy manure and different carbon amendments.  J. Food Prot.  77:1911-1918.

Erickson, M.C., C.C. Webb, J.C. Diaz-Perez, L.E. Davey, A.S. Payton, I.D. Flitcroft, S.C. Phatak, and M.P. Doyle. 2014. Absence of internalization of Escherichia coli O157:H7 into germinating tissue of field-grown leafy greens. J. Food Prot. 77:189-196.

Erickson, M.C., C.C. Webb, L.E. Davey, A.S. Payton, I.D. Flitcroft, and M.P. Doyle. 2014. Internalization and fate of Escherichia coli O157:H7 in leafy green phyllosphere tissue using various spray conditions. J. Food Prot. 77:713-721.

Erickson, M.C., C.C. Webb, L.E. Davey, A.S. Payton, I.D. Flitcroft, and M.P. Doyle. 2014. Biotic and abiotic variables affecting internalization and fate of Escherichia coli O157:H7 isolates in leafy green roots. J. Food Prot. 77:872-879.

Newell, C.R., M. Doyle, and L. Ma. 2015. Inability of non-proteolytic Clostridium botulinum to grow in mussels inoculated via immersion and packaged in high oxygen atmospheres.  Food Microbiol. 46:204-209.

Ta, Y.T., T.T. Nguyen, P.B. To, D.X. Pham, H.T.H. Le, G.N. Thi, W.Q. Alali, I. Walls, and M.P. Doyle. 2014. Quantification, serovars, and antibiotic resistance of Salmonella isolated from retail raw chicken meat in Vietnam .  J. Food Prot. 77:57-66.

Webb, C.C., M.C. Erickson, L.E. Davey, A.S. Payton, and M.P. Doyle. 2014. Construction and characterization of outbreak Escherichia coli O157:H7 surrogate strains for use in field studies.  Foodborne Path. Dis.  11:893-899.

Yang, B.W., Y. Cui, C. Shi, J.Q. Wang, X.D. Xia, M.L. Xi, X. Wang, J.H. Meng, W.Q. Alali, I. Walls, and M.P. Doyle. 2014, Counts, serotypes, and antimicrobial resistance of Salmonella isolates on retail raw poultry in the People's Republic of China. J. Food Prot. 77:894-902.

Zhao, T., P. Zhao, D. Chen, R. Jadeja, Y.C. Hung, and M.P. Doyle. 2014. Reductions of Shiga toxin-producing Escherichia coli and Salmonella Typhimurium on beef trim by lactic acid, levulinic acid, and sodium dodecyl sulfate treatments. J. Food Prot. 77:528-537.

Erickson, M.C., C.C. Webb, J.C. Diaz-Perez, L.E. Davey, A.S. Payton, I.D. Flitcroft, S.C. Phatak, and M.P. Doyle. 2013. Internalization of Escherichia coli O157:H7 following spraying of cut shoots when leafy greens are regrown for a second crop. J. Food Prot. 76:2052-2056.

Aydin, A., J.L. Cannon, T. Zhao, and M.P. Doyle. 2013. Efficacy of a levulinic acid plus sodium dodecyl sulfate (SDS)-based sanitizer on inactivation of influenza A virus on eggshells.  Food Environ. Virol. 5:215-219.

Doyle, M.P., G.H. Loneragan, H.M. Scott, and R.S. Singer. 2013. Antimicrobial resistance: Challenges and perspectives.  Compr. Rev. Food Sci. Food Safety 12:234-248.

Doyle, M.P., G.H. Loneragan, H.M. Scott, and R.S. Singer. 2013. Antimicrobial resistance and food animals.  Food Technol. 67(3):42-44.

Kendall, M.E., R.K. Mody, B.E. Mahon, M.P. Doyle, K.M. Herman, and R.V. Tauxe. 2013. Emergence of salsa and guacamole as frequent vehicles of foodborne disease outbreaks in the United States, 1973-2008. Foodborne Path. Dis. 10:316-322.

Erickson, M.C., J. Liao, A.S. Payton, C.C. Webb, L. Ma, G. Zhang, I. Flitcroft, M.P. Doyle, and L.R. Beuchat. 2013. Fate of Escherichia coli O157:H7 and Salmonella in soil and lettuce roots as affected by potential home gardening practices. J. Sci. Food Agric. 93:3841-3849.

Webb, C.C., L.E. Davey, M. Erickson, and M.P. Doyle. 2013. Evaluation of levulinic acid and sodium dodecyl sulfate as a sanitizer for use in processing Georgia-grown cantaloupes. J. Food Prot. 76:1767-1772.

Alali, W.Q., C. L. Hofacre, G. F. Mathis, G. Faltys, S. C. Ricke, M. P. Doyle. 2013. Effect of non-pharmaceutical compounds on shedding and colonization of Salmonella enterica serovar Heidelberg in broilers. Food Control. 31: 125-128.  

Zhao, T., T. Podtburg, P. Zhao, D. Chen, D.A. Baker, B. Cords, and M.P. Doyle. 2013. Reduction by competitive bacteria of Listeria monocytogenes in biofilms and Listeria sp. in floor drains in a ready-to-eat poultry processing plant. J. Food Prot. 76:601-607.

Zhao, T., P. Zhao, and M.P. Doyle. 2012. Detection and isolation of Yersinia pestis without fraction-1 antigen by monoclonal antibody in foods and water. J. Food Prot. 75:1555-1561.

Alali, W.Q., R. Gaydashov, E. Petrova, A. Panin, O. Tugarinov, A. Kulikovskii, D. Mamleeva, I. Walls, and M.P. Doyle. 2012. Prevalence of Salmonella on retail chicken meat in Russian federation .  J. Food Prot. 75:1469-1473.

Cannon, J.L., A. Aydin, A.N. Mann, S.L. Bolton, T. Zhao, and M.P. Doyle. 2012. Efficacy of a levulinic acid plus sodium dodecyl sulfate-based sanitizer on inactivation of human norovirus surrogates.  J. Food Prot. 75:1532-1535.

Cox, N.A., L.J. Richardson, J.J. Mauer, M.E. Berrang, P.J. Fedorka-Cray, R.J. Buhr, J.A. Byrd, M.D. Lee, C.L. Hofacre, P.M. O'Kane, A.M. Lammerding, A.G. Clark, S.G. Thayer, and M.P. Doyle. 2012. Evidence for horizontal and vertical transmission in Campylobacter passage from hen to her progency.  J. Food Prot. 75:1896-1902.

Donado-Godoy, P., V. Clavijo, M. Leon, M. Tafur, S. Gonzales, M. Hume, W. Alali, I. Walls, D.M.A.L.F. Wong, and M.P. Doyle. 2012. Prevalence of Salmonella on retail broiler chicken meat carcasses in Colombia .  J. Food Prot. 75:1134-1138.

Doyle, M.P. and M.C. Erickson. 2012. Opportunities for mitigating pathogen contamination during on-farm food production.  Int. J. Food Microbiol. 152:54-74.

Newell, C.R., L. Ma, and M. Doyle. 2012. Botulism challenge studies of a modified atmosphere package for fresh mussels: Inoculated pack studies.  J. Food Prot. 75:1157-1166.

Ta., Y.T., T.T. Nguyen, P.B. To, D.X. Pham, T.H.L. Hao, W.Q. Alali, I. Walls, D.M.A.L.F. Wong, and M.P. Doyle. 2012. Prevalence of Salmonella on chicken carcasses from retail markets in Vietnam .  J. Food Prot. 75:1851-1854.

Wang, B. Y., J. Hong, S. G. Ciancio, T. Zhao, and M. P. Doyle. 2012.  A novel formulation effective in killing oral biofilm bacteria. J. Int. Acad. Periodontology. 14:56-61.

Wang, H., J.C. McEntire, L. Zhang, X. Li, and M. Doyle. 2012. The transfer of antibiotic resistance from food to humans: facts, implications and future directions.  Rev. Sci. Tech.Off. Int. Epiz. 31:249-260.

Yang, B.W., M.L. Xi., X. Wang, S.H. Cui, T.L. Yue, H.S. Hao, Y. Wang, Y. Cui, W.Q. Alali, J.H. Meng, I. Walls, D.M.L. Wong, and M.P. Doyle. 2011. Prevalence of Salmonella on raw poultry at retail markets in China .  J. Food Prot. 74:1724-1728.

Zhao, T., P. Zhao, J.L. Cannon, and M.P. Doyle. 2011. Inactivation of Salmonella in biofilms and on chicken cages and preharvest poultry by levulinic acid and sodium dodecyl sulfate.  J. Food Prot. 74:2024-2030.

Shepherd, M.W., J. Kim, X.P. Jiang, M.P. Doyle, and M.C. Erickson. 2011. Evaluation of physical coverings used to control Escherichia coli O157:H7 at the compost heap surface. Appl. Environ. Microbiol. 77:5044-5049.

Ma, L., G.D. Zhang, and M.P. Doyle. 2011. Green fluorescent protein labelling of Listeria, Salmonella, and Escherichia coli O157:H7 for safety-related studies. PLoS One 4:e18083.

Erickson, M.C., C.C. Webb, J.C. Diaz-Perez, S.C. Phatak, J.J. Silvoy, L. Davey, A.S. Payton, J. Liao, L. Ma, and M.P. Doyle. 2010. Infrequent internalization of Escherichia coli O157:H7 into field-grown leafy greens. J. Food Prot. 73:500-506.

Erickson, M.C., C.C. Webb, J.C. Diaz-Perez, S.C. Phatak, J.J. Silvoy, L. Davey, A.S. Payton, J. Liao, L. Ma, and M.P. Doyle. 2010. Surface and internalized Escherichia coli O157:H7 on field-grown spinach and lettuce treated with spray-contaminated irrigation water. J. Food Prot. 73:1023-1029.

Erickson, M.C., J. Liao, G. Boyhan, C. Smith, L. Ma, X. Jiang, and M.P. Doyle. 2010. Fate of manure-borne pathogen surrogates in static composting piles of chicken litter and peanut hulls.  Bioresource Technol. 101:1014-1020.

Erickson, M.C., J. Liao, A.S. Payton, D.G. Riley, C.C. Webb, L.E. Davey, S. Kimbrel, L. Ma, G.D. Zhang, I. Flitcroft, M.P. Doyle, and L.R. Beuchat. 2010. Preharvest internalization of Eschcerichia coli O157:H7 into lettuce leaves, as affected by insect and physical damage. J. Food Prot. 73:1809-1816.

Heringa, S.D., J. Kim, X.P. Jiang, M.P. Doyle, and M.C. Erickson. 2010. Use of a mixture of bacteriophages for biological control of Salmonella enterica strains in compost. Appl. Environ. Microbiol. 76:5327-5332.

Shepherd, M., P. Liang, X. Jiang, M.P. Doyle, and M.C. Erickson. 2010. Microbiological analysis of composts produced on South Carolina poultry farms.  J. Appl. Microbiol. 108:2067-2076.

Cox, N.A., L.J. Richardson, J.A. Cason, R.J. Buhr, Y. Vizzier-Thaxton, D.P. Smith, P.J. Fedorka-Cray, C.P. Romanenghi, L.V.B. Pereira, and M.P. Doyle. 2010. Comparison of neck skin excision and whole carcass rinse sampling methods for microbiological evaluation of broiler carcasses before and after immersion chilling. J. Food Prot. 73:976-980.

Critzer, F.J. and M.P. Doyle. 2010. Microbial ecology of foodborne pathogens associated with produce. Curr. Opin. Biotechnol. 21:125-130.

Dorea, F.C., D.J. Cole, C. Hofacre, K. Zamperini, D. Mathis, M.P. Doyle, M.D. Lee, and J.J. Maurer. 2010. Effect of Salmonella vaccination of breeder chickens on contaminaiton of broiler chicken carcasses in integrated poultry operations. Appl. Environ. Microbiol. 76:7820-7825.

Ma, L., G.D. Zhang, P. Gerner-Smidt, R.V. Tauxe, and M.P. Doyle. 2010. Survival and growth of Salmonella in salsa and related ingredients. J. Food Prot. 73:434-444.

Mead, G., A.M. Lammerding, N. Cox, M.P. Doyle, F. Humbert, A. Kulikovsky, A. Panin, V.P. do Nascimento, and M. Wierup. 2010. Scientific and technical factors affecting the setting of Salmonella criteria for raw poultry: Global pespective. J. Food Prot. 73:1566-1590.

Tauxe, R.V., M.P. Doyle, T. Kuchenmueller, J. Schlundt, and C.E. Stein. 2010. Evolving public health approaches to the global challenge of foodborne infections. Int. J. Food Microbiol. 139:S16-S28.

Zhao, T., P. Zhao, and M.P. Doyle. 2010. Inactivation of Escherichia coli O157:H7 and Salmonella Typhimurium DT104 on alfalfa seeds by levulinic acid and sodium dodecyl sulfate. J. Food Prot. 73:2010-2017.

Zhang, G., L. Ma, L.R. Beuchat, M.C. Erickson, V.H. Phelan, and M.P. Doyle. 2009. Heat and drought stress during growth of lettuce (Lactuca sativa L.) does not promote internalization of Escherichia coli O157:H7.  J. Food Prot. 72:2471-2475.

Zhang, G., L. Ma, L.R. Beuchat, M.C. Erickson, V.H. Phelan, and M.P. Doyle. 2009. Lack of internalization of Escherichia coli O157:H7 in lettuce (Lactuca sativa L.) after leaf surface and soil inoculation.  J. Food Prot. 72:2028-2037.

Zhang, G.D., L. Ma, L.R. Beuchat, M.C. Erickson, V.H. Phelan, and M.P. Doyle. 2009. Evaluation of treatments for elimination of foodborne pathogens on the surface of leaves and roots of lettuce (Lactuca sativa L.).  J. Food Prot. 72:228-234.

Erickson, M.C., J. Liao, L. Ma, X. Jiang, and M.P. Doyle. 2009. Inactivation of Salmonella spp. in cow manure composts formulated to different initial C:N ratios. Bioresource Technol. 100:5898-5903.

Taormina , P.J., L.R. Beuchat, M.C. Erickson, L. Ma, G.D. Zhang, and M.P. Doyle. 2009. Transfer of Escherichia coli O157:H7 to iceberg lettuce via simulated field coring.  J. Food Prot. 72:465-472.

Erickson, M.C., J. Liao, L. Ma, X.P. Jiang, and M.P. Doyle. 2009. Pathogen inactivation in cow manure compost. Compost Sci. Util. 17:229-236.

Ma, L., G. Zhang, P. Gerner-Smidt, V. Mantripragada, K. Ezeoke, and M.P. Doyle. 2009. Thermal inactivation of Salmonella in peanut butter. J. Food Prot. 72:1596-1601.

Zhang, G., L. Ma, V.H. Phelan, and M.P. Doyle. 2009. Efficacy of antimicrobial agents in lettuce leaf processing water for control of Escherichia coli O157:H7. J. Food Prot. 72:1392-1397.

Zhao, T., P. Zhao, and M.P. Doyle. 2009. Inactivation of Salmonella and Escherichia coli O157:H7 on lettuce and poultry skin by combinations of levulinic acid and sodium dodecyl sulfate. J. Food Prot. 72:928-936.

Taormina , P.J., L.R. Beuchat, M.C. Erickson, L. Ma, G.D. Zhang, and M.P. Doyle. 2009. Transfer of Escherichia coli O157:H7 to iceberg lettuce via simulated field coring.  J. Food Prot. 72:465-472.

Zhang, G.D., L. Ma, L.R. Beuchat, M.C. Erickson, V.H. Phelan, and M.P. Doyle. 2009. Evaluation of treatments for elimination of foodborne pathogens on the surface of leaves and roots of lettuce (Lactuca sativa L.).  J. Food Prot. 72:228-234.

Ma, L., G.D. Zhang, B. Swaminathan, M. Doyle, and A. Bowen. 2009. Efficacy of protocols for cleaning an disinfecting infant feeding bottles in less developed communitieis. Am. J. Trop. Med. Hyg. 81:132-139.

Doyle, M.P. 2008. From wild pigs and spinach to tilapia and Asia : The challenges of the food safety community (The John H. Silliker Lecture).  Food Prot. Trends 28:800-803.

Doyle, M.P. and M.C. Erickson. 2008. Summer meeting 2007 – the problems with fresh produce: an overview.  J. Appl. Microbiol. 105:317-330.

Smith, M.A., K. Takeuchi, G. Anderson, G.O. Ware, H.M. McClure, R.B. Raybourne, N. Mytle, and M.P. Doyle. 2008. Dose-response model for Listeria monocytogenes-induced stillbirths in nonhuman primates.  Infect. Immun. 76:726-731.